质粒名称: | pBABEpuro HA Brca1 |
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出品公司: | 爱迪基因 |
目录编号: | 14999 |
存储实验室: | Stephen Elledge |
载体骨架基本信息 |
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载体名称: | pBABE-puro |
载体出品公司: | -- |
载体抗性: | 氨苄青霉素 |
空载体大小: | -- |
完整质粒大小: | 5200 bp |
载体修饰: | 人细胞色素c氧化酶亚基VIII(human cytochrome C oxidase subunit VIII) |
载体类型: | 哺乳细胞表达 |
筛选标记: | 新霉素(G418) |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | Brca1 |
物种来源: | -- |
插入大小: | -- |
基因突变: | -- |
GenBank编号 | -- |
启动子 | -- |
标签/融合蛋白: | 人细胞色素c氧化酶亚基VIII(N端) |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | AgeI(未破坏) |
3' 克隆位点: | NotI (未破坏) |
5' 测序引物 | CMV-F |
3' 测序引物 | SV40pA-R |
文献引用方法 |
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材料和方法部分: | pBABEpuro HA Brca1 was a gift from Stephen Elledge (Addgene plasmid # 14999 ; http://n2t.net/addgene:14999 ; RRID:Addgene_14999) |
参考文献部分: | Requirement of ATM-dependent phosphorylation of brca1 in the DNA damage response to double-strand breaks. Cortez D, Wang Y, Qin J, Elledge SJ. Science. 1999 Nov 5. 286(5442):1162-6. 10.1126/science.286.5442.1162 PubMed 10550055 |
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载体基本信息
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载体简介
载体序列
LOCUS pBABEpuro HA Brca1 10749 bp ds-DNA circular SYN 06-AUG-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pBABEpuro HA Brca1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10749) AUTHORS Cortez D, Wang Y, Qin J, Elledge SJ TITLE Requirement of ATM-dependent phosphorylation of brca1 in the DNA damage response to double-strand breaks. JOURNAL Science. 1999 Nov 5. 286(5442):1162-6. PUBMED 10550055 REFERENCE 2 (bases 1 to 10749) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10749 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(11..28) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(182..770) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(262..281) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(930..1790) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1553..1572 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1791..1895) /gene="bla" /label=AmpR promoter LTR 1926..2395 /note="long terminal repeat from Moloney murine leukemia virus" misc_feature 2460..2659 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 2860..3276 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 3206..3228 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 3244..3260 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" CDS 3347..3373 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" primer_bind complement(8997..9017) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 9002..9331 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 9182..9317 /label=SV40 ori /note="SV40 origin of replication" primer_bind 9244..9263 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 9341..9940 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(9341..9360) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 9837..9857 /label=Puro-F /note="Puromycin resistance gene, forward primer" LTR 10161..10630 /note="long terminal repeat from Moloney murine leukemia virus" ORIGIN 1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta 661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 841 tcatgagatt atcaaaaagg atcttcacct agatcctttt gcggccgcaa atcaatctaa 901 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 961 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 1021 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 1081 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 1141 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 1201 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 1261 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 1321 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 1381 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 1441 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 1501 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 1561 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 1621 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 1681 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 1741 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 1801 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 1861 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 1921 gcagcctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg 1981 gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc 2041 ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt 2101 tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt 2161 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc 2221 tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc 2281 ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg 2341 ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttgg 2401 gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca ccgggaggca 2461 agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgac tgattttatg 2521 cgcctgcgtc ggtactagtt agctaactag ctctgtatct ggcggacccg tggtggaact 2581 gacgagttct gaacacccgg ccgcaaccct gggagacgtc ccagggactt tgggggccgt 2641 ttttgtggcc cgacctgagg aagggagtcg atgtggaatc cgaccccgtc aggatatgtg 2701 gttctggtag gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg 2761 gtttggaacc gaagccgcgc gtcttgtctg ctgcagcgct gcagcatcgt tctgtgttgt 2821 ctctgtctga ctgtgtttct gtatttgtct gaaaattagg gccagactgt taccactccc 2881 ttaagtttga ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta 2941 gatgtcaaga agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc 3001 ggatggccgc gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc 3061 ttttcacctg gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc 3121 ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct 3181 cttcctccat ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc 3241 tccctttatc cagccctcac tccttctcta ggcgccggcc ggatcccagt gtggtggtac 3301 gtaggaattc gccagcacag tggtcgaccc caagcttacc atggcctacc cctacgacgt 3361 gcccgactac gcctccctcg gatccatgga tttatctgct cttcgcgttg aagaagtaca 3421 aaatgtcatt aatgctatgc agaaaatctt agagtgtccc atctgtctgg agttgatcaa 3481 ggaacctgtc tccacaaagt gtgaccacat attttgcaaa ttttgcatgc tgaaacttct 3541 caaccagaag aaagggcctt cacagtgtcc tttatgtaag aatgatataa ccaaaaggag 3601 cctacaagaa agtacgagat ttagtcaact tgttgaagag ctattgaaaa tcatttgtgc 3661 ttttcagctt gacacaggtt tggagtatgc aaacagctat aattttgcaa aaaaggaaaa 3721 taactctcct gaacatctaa aagatgaagt ttctatcatc caaagtatgg gctacagaaa 3781 ccgtgccaaa agacttctac agagtgaacc cgaaaatcct tccttgcagg aaaccagtct 3841 cagtgtccaa ctctctaacc ttggaactgt gagaactctg aggacaaagc agcggataca 3901 acctcaaaag acgtctgtct acattgaatt gggatctgat tcttctgaag ataccgttaa 3961 taaggcaact tattgcagtg tgggagatca agaattgtta caaatcaccc ctcaaggaac 4021 cagggatgaa atcagtttgg attctgcaaa aaaggctgct tgtgaatttt ctgagacgga 4081 tgtaacaaat actgaacatc atcaacccag taataatgat ttgaacacca ctgagaagcg 4141 tgcagctgag aggcatccag aaaagtatca gggtagttct gtttcaaact tgcatgtgga 4201 gccatgtggc acaaatactc atgccagctc attacagcat gagaacagca gtttattact 4261 cactaaagac agaatgaatg tagaaaaggc tgaattctgt aataaaagca aacagcctgg 4321 cttagcaagg agccaacata acagatgggc tggaagtaag gaaacatgta atgataggcg 4381 gactcccagc acagaaaaaa aggtagatct gaatgctgat cccctgtgtg agagaaaaga 4441 atggaataag cagaaactgc catgctcaga gaatcctaga gatactgaag atgttccttg 4501 gataacacta aatagcagca ttcagaaagt taatgagtgg ttttccagaa gtgatgaact 4561 gttaggttct gatgactcac atgatgggga gtctgaatca aatgccaaag tagctgatgt 4621 attggacgtt ctaaatgagg tagatgaata ttctggttct tcagagaaaa tagacttact 4681 ggccagtgat cctcatgagg ctttaatatg taaaagtgaa agagttcact ccaaatcagt 4741 agagagtaat attgaagaca aaatatttgg gaaaacctat cggaagaagg caagcctccc 4801 caacttaagc catgtaactg aaaatctaat tataggagca tttgttactg agccacagat 4861 aatacaagag cgtcccctca caaataaatt aaagcgtaaa aggagaccta catcaggcct 4921 tcatcctgag gattttatca agaaagcaga tttggcagtt caaaagactc ctgaaatgat 4981 aaatcaggga actaaccaaa cggagcagaa tggtcaagtg atgaatatta ctaatagtgg 5041 tcatgagaat aaaacaaaag gtgattctat tcagaatgag aaaaatccta acccaataga 5101 atcactcgaa aaagaatctg ctttcaaaac gaaagctgaa cctataagca gcagtataag 5161 caatatggaa ctcgaattaa atatccacaa ttcaaaagca cctaaaaaga ataggctgag 5221 gaggaagtct tctaccaggc atattcatgc gcttgaacta gtagtcagta gaaatctaag 5281 cccacctaat tgtactgaat tgcaaattga tagttgttct agcagtgaag agataaagaa 5341 aaaaaagtac aaccaaatgc cagtcaggca cagcagaaac ctacaactca tggaaggtaa 5401 agaacctgca actggagcca agaagagtaa caagccaaat gaacagacaa gtaaaagaca 5461 tgacagtgat actttcccag agctgaagtt aacaaatgca cctggttctt ttactaagtg 5521 ttcaaatacc agtgaactta aagaatttgt caatcctagc cttccaagag aagaaaaaga 5581 agagaaacta gaaacagtta aagtgtctaa taatgctgaa gaccccaaag atctcatgtt 5641 aagtggagaa agggttttgc aaactgaaag atctgtagag agtagcagta tttcactggt 5701 acctggtact gattatggca ctcaggaaag tatctcgtta ctggaagtta gcactctagg 5761 gaaggcaaaa acagaaccaa ataaatgtgt gagtcagtgt gcagcatttg aaaaccccaa 5821 gggactaatt catggttgtt ccaaagataa tagaaatgac acagaaggct ttaagtatcc 5881 attgggacat gaagttaacc acagtcggga aacaagcata gaaatggaag aaagtgaact 5941 tgatgctcag tatttgcaga atacattcaa ggtttcaaag cgccagtcat ttgctctgtt 6001 ttcaaatcca ggaaatgcag aagaggaatg tgcaacattc tctgcccact ctgggtcctt 6061 aaagaaacaa agtccaaaag tcacttttga atgtgaacaa aaggaagaaa atcaaggaaa 6121 gaatgagtct aatatcaagc ctgtacagac agttaatatc actgcaggct ttcctgtggt 6181 tggtcagaaa gataagccag ttgataatgc caaatgtagt atcaaaggag gctctaggtt 6241 ttgtctatca tctcagttca gaggcaacga aactggactc attactccaa ataaacatgg 6301 acttttacaa aacccatatc gtataccacc actttttccc atcaagtcat ttgttaaaac 6361 taaatgtaag aaaaatctgc tagaggaaaa ctttgaggaa cattcaatgt cacctgaaag 6421 agaaatggga aatgagaaca ttccaagtac agtgagcaca attagccgta ataacattag 6481 agaaaatgtt tttaaaggag ccagctcaag caatattaat gaagtaggtt ccagtactaa 6541 tgaagtgggc tccagtatta atgaaatagg ttccagtgat gaaaacattc aagcagaact 6601 aggtagaaac agagggccaa aattgaatgc tatgcttaga ttaggggttt tgcaacctga 6661 ggtctataaa caaagtcttc ctggaagtaa ttgtaagcat cctgaaataa aaaagcaaga 6721 atatgaagaa gtagttcaga ctgttaatac agatttctct ccatatctga tttcagataa 6781 cttagaacag cctatgggaa gtagtcatgc atctcaggtt tgttctgaga cacctgatga 6841 cctgttagat gatggtgaaa taaaggaaga tactagtttt gctgaaaatg acattaagga 6901 aagttctgct gtttttagca aaagcgtcca gagaggagag cttagcagga gtcctagccc 6961 tttcacccat acacatttgg ctcagggtta ccgaagaggg gccaagaaat tagagtcctc 7021 agaagagaac ttatctagtg aggatgaaga gcttccctgc ttccaacact tgttatttgg 7081 taaagtaaac aatatacctt ctcagtctac taggcatagc accgttgcta ccgagtgtct 7141 gtctaagaac acagaggaga atttattatc attgaagaat agcttaaatg actgcagtaa 7201 ccaggtaata ttggcaaagg catctcagga acatcacctt agtgaggaaa caaaatgttc 7261 tgctagcttg ttttcttcac agtgcagtga attggaagac ttgactgcaa atacaaacac 7321 ccaggatcct ttcttgattg gttcttccaa acaaatgagg catcagtctg aaagccaggg 7381 agttggtctg agtgacaagg aattggtttc agatgatgaa gaaagaggaa cgggcttgga 7441 agaaaataat caagaagagc aaagcatgga ttcaaactta ggtgaagcag catctgggtg 7501 tgagagtgaa acaagcgtct ctgaagactg ctcagggcta tcctctcaga gtgacatttt 7561 aaccactcag cagagggata ccatgcaaca taacctgata aagctccagc aggaaatggc 7621 tgaactagaa gctgtgttag aacagcatgg gagccagcct tctaacagct acccttccat 7681 cataagtgac tcctctgccc ttgaggacct gcgaaatcca gaacaaagca catcagaaaa 7741 agcagtatta acttcacaga aaagtagtga ataccctata agccagaatc cagaaggcct 7801 ttctgctgac aagtttgagg tgtctgcaga tagttctacc agtaaaaata aagaaccagg 7861 agtggaaagg tcatcccctt ctaaatgccc atcattagat gataggtggt acatgcacag 7921 ttgctctggg agtcttcaga atagaaacta cccatctcaa gaggagctca ttaaggttgt 7981 tgatgtggag gagcaacagc tggaagagtc tgggccacac gatttgacgg aaacatctta 8041 cttgccaagg caagatctag agggaacccc ttacctggaa tctggaatca gcctcttctc 8101 tgatgaccct gaatctgatc cttctgaaga cagagcccca gagtcagctc gtgttggcaa 8161 cataccatct tcaacctctg cattgaaagt tccccaattg aaagttgcag aatctgccca 8221 gggtccagct gctgctcata ctactgatac tgctgggtat aatgcaatgg aagaaagtgt 8281 gagcagggag aagccagaat tgacagcttc aacagaaagg gtcaacaaaa gaatgtccat 8341 ggtggtgtct ggcctgaccc cagaagaatt tatgctcgtg tacaagtttg ccagaaaaca 8401 ccacatcact ttaactaatc taattactga agagactact catgttgtta tgaaaacaga 8461 tgctgagttt gtgtgtgaac ggacactgaa atattttcta ggaattgcgg gaggaaaatg 8521 ggtagttagc tatttctggg tgacccagtc tattaaagaa agaaaaatgc tgaatgagca 8581 tgattttgaa gtcagaggag atgtggtcaa tggaagaaac caccaaggtc caaagcgagc 8641 aagagaatcc caggacagaa agatcttcag ggggctagaa atctgttgct atgggccctt 8701 caccaacatg cccacagatc aactggaatg gatggtacag ctgtgtggtg cttctgtggt 8761 gaaggagctt tcatcattca cccttggcac aggtgtccac ccaattgtgg ttgtgcagcc 8821 agatgcctgg acagaggaca atggcttcca tgcaattggg cagatgtgtg aggcacctgt 8881 ggtgacccga gagtgggtgt tggacagtgt agcactctac cagtgccagg agctggacac 8941 ctacctgata ccccagatcc cccacagcca ctactgagat atcggctcga ccctgtggaa 9001 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag 9061 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag 9121 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc 9181 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 9241 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg 9301 aggctttttt ggaggcctag gcttttgcaa aaagcttacc atgaccgagt acaagcccac 9361 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt 9421 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac 9481 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc 9541 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt 9601 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca 9661 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac 9721 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg 9781 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa 9841 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg 9901 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca 9961 gcgcccgacc gaaaggagcg cacgacccca tgcatcgata aaataaaaga ttttatttag 10021 tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt 10081 aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagagaagt tcagatcaag 10141 gtcaggaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc 10201 ctgccccggc tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc 10261 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc 10321 cagccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa 10381 atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc 10441 ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc 10501 gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga 10561 cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg 10621 gggtctttca catgcagcat gtatcaaaat taatttggtt ttttttctta agtatttaca 10681 ttaaatggcc atagttgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 10741 ttgggcgct //
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